搜索
中压柱层析法分离白藜芦醇的研究
2013-03-19 16:03:00 益择发布X
SU W Q
苏文强1 , 杨 磊1 , 朱明华1 , 陈启凡2 , 刘文彬3
(1. 东北林业大学材料科学与工程学院, 黑龙江哈尔滨150040 ;
2. 辽东学院试验中心, 辽宁丹东118003 ;
3. 哈尔滨工程大学化工系, 黑龙江哈尔滨150001)
摘 要: 对虎杖( Polygonum cuspidatum Sieb. et Zucc. ) 中的白藜芦醇进行了提取分离和含量测定。考察了浸
提温度、浸提液浓度、浸提时间和次数、料液比等5 个因素对白藜芦醇提取的影响,确立了白藜芦醇最佳提取
条件为:浸提液体积分数为95 % ,浸提温度60 ℃,料液比11∶ 1 ,回流提取3 次,每次60 min。采用中压硅胶柱层
析法分离白藜芦醇,产物经重结晶后用高效液相色谱测定含量达99. 28 %。结果表明所建立的制备及分离纯
化工艺可用于白藜芦醇的生产。
关键词: 白藜芦醇;虎杖;正交试验;中压柱层析
中图分类号:O658. 11 ; Q949. 744 文献标识码:A 文章编号:0253 - 2417(2004) 01 - 0039 - 04
STUDIES ON EXTRACTION OF RESVERATROL FROM
POLYGONUM CUSPIDATUM BYMEDIUM2PRESSURE
SILICA GEL COLUMN CHROMATOGRAPHY
SU Wen2qiang1 , YANGLei1 , ZHU Ming2hua1 , CHEN Qi2fan2 , LIU Wen2bin3
(1. College of Material Science and Engineering , Northeast Forestry University , Harbin 150040 , China ;
2. Experiment Center of Liaodong College , Dandong 118003 , China ;3. Department of
Chemical Engineering , Harbin Engineering University , Harbin 150001 , China)
Abstract : Extraction ,separation ,structure identification and content determination of resveratrol from Polygonum cuspidatum
Sieb. et Zucc. had been carried out. Orthogonal experiment indicated that optimum extraction condition of resveratrol was :60
℃,95 % ethanol 1∶ 11 (raw material∶ ethanol) ,60 min and 3 times of extraction. Separation experiments with silica gel column
under about 3 MPa pressure showed that ethyl acetate :petroleum ether was the ideal eluant. After recrystallizing , resveratrol
content was determined by HPLC to be as high as 99. 28 %. It can be demonstrated that this is a good technique used in
resveratrol manufacture.
Key words :resveratrol ; Polygonum cuspidatum Sieb. et Zucc. ; orthogonal experiment ; medium2pressure column chro2
相关阅读
暂无评论
- 网友评论:
- 已有0条评论
